Related Experiment Video
Updated: Oct 3, 2026

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Nox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidases
1Department of Pathology, Emory University Medical School, Atlanta, Georgia 30322, USA. dlambe@emory.edu
Abstract:
Reactive oxygen species are classically described as occurring as an accidental byproduct of respiration, and are generally thought to be deleterious to biologic systems. The phagocyte nicotinamide adenine dinucleotide phosphate oxidase provides an example of deliberate reactive oxygen species generation, but the function of this enzyme is to oxidatively modify bacteria as part of bactericidal mechanisms. The discovery of a family of nicotinamide adenine dinucleotide (phosphate) oxidases related to the phagocyte oxidase, the Nox/Duox family, provides additional examples of deliberate generation of reactive oxygen species. This article describes this new family of enzymes and considers hypotheses for their function. Potential roles of Nox/Duox in generation of reactive oxygen species that function in cell signaling (related to growth and angiogenesis), immune function, hypoxic response, and oxidative modification of extracellular matrix proteins are discussed.
Related Concept Videos
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Role of Reduced Coenzymes NADH and FADH₂
Electron Transport Chain: Complex III and IV
Oxidation of Phenols to Quinones
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Redox Reactions
