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Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
Published on: February 12, 2016
Changes in phagocyte activity in patients with ischaemic stroke
M L Alexandrova1, P G Bochev, V I Markova
1Department of Biophysics, Medical University, I Kliment Ohridski Str., 5800 Pleven, Bulgaria. margalexandrova@hotmail.com
Luminescence : the Journal of Biological and Chemical Luminescence
|December 26, 2001
Summary
Peripheral phagocytes in stroke patients show heightened reactive oxygen species (ROS) production. This enhanced extracellular ROS generation, particularly in severe cases, may serve as a biomarker for oxidative stress in cerebral ischemia.
Area of Science:
- Immunology
- Neurology
- Biochemistry
Background:
- Peripheral phagocyte activity is crucial in inflammatory responses.
- Ischaemic stroke triggers complex immune system alterations.
- Reactive oxygen species (ROS) play a dual role in cellular function and pathology.
Purpose of the Study:
- To investigate peripheral phagocyte ROS generation in ischaemic stroke patients.
- To correlate phagocyte activity with stroke severity and time since onset.
- To identify potential biomarkers for oxidative stress in cerebral ischemia.
Main Methods:
- Evaluation of total and extracellular ROS production using luminol chemiluminescence.
- Assessment of plasma oxidant activity.
- Stimulation of phagocytes with opsonized zymosan and formyl-methionyl-leucyl-phenylalanine.
Main Results:
- Phagocytes in stroke patients exhibited rapid activation upon stimulation.
- Total ROS generation increased over time in all stroke cases.
- Extracellular ROS generation was significantly higher in severe stroke cases.
- Plasma oxidant activity was enhanced in patients with ischaemic stroke.
Conclusions:
- Circulating phagocytes in ischaemic stroke are primed for enhanced ROS production.
- Enhanced extracellular ROS generation via opsonin receptor-dependent stimulation is a potential biomarker for cerebral ischemia.
- Myeloperoxidase secretion is increased via opsonin receptor-independent stimulation.
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Overview
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