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Updated: Oct 3, 2026

In vitro Measurements of Tracheal Constriction Using Mice
Published on: June 25, 2012
RANTES release by human airway smooth muscle: effects of prostaglandin E(2) and fenoterol
N Lazzeri1, M G Belvisi, H J Patel
1Thoracic Medicine, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY, UK.
Abstract:
In human airway smooth muscle cells, the levels of RANTES were increased upon stimulation with interleukin-1beta together with tumour necrosis factor-alpha (TNF-alpha) (10 ng ml(-1) for each). In this study, we have assessed the effects of prostaglandin E(2) and the beta(2)-adrenoceptor agonist, fenoterol on RANTES (regulated upon activation, normal T cell expressed and secreted) release by these cells. The levels of RANTES released by human airway smooth muscle cells were measured after 24 h of treatment. Prostaglandin E(2) and fenoterol, only in presence of a cyclo-oxygenase inhibitor indomethacin (10(-6) M), provoked a concentration-dependent reduction in RANTES release. These data suggest that, in settings where cyclo-oxygenase activity is low, both drugs may relieve the symptoms of airway diseases by reducing RANTES production.
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