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Related Experiment Videos

Intracellular flavonoids as electron donors for extracellular ferricyanide reduction in human erythrocytes.

Mara Fiorani1, Roberta De Sanctis, Roberta De Bellis

  • 1Giorgio Fornaini Institute of Biological Chemistry, Università degli Studi di Urbino, Via Saffi 2, 60129 Urbino, Italy. m.fiorani@uniurb.it

Free Radical Biology & Medicine
|January 5, 2002
PubMed
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Flavonoids quercetin and myricetin stimulate ferricyanide reduction in red blood cells, acting as intracellular donors for a trans-plasma membrane oxidoreductase. This suggests a new way these compounds may combat oxidative stress.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Trans-plasma membrane oxidoreductases in human red blood cells reduce extracellular ferricyanide, using ascorbic acid as an electron donor.
  • Flavonoids are plant compounds with potential health benefits, but their specific roles in cellular redox processes are not fully understood.

Purpose of the Study:

  • To investigate the effect of flavonoids quercetin and myricetin on the trans-plasma membrane oxidoreductase activity in human red blood cells.
  • To determine if quercetin and myricetin can act as alternative electron donors for this enzyme.

Main Methods:

  • Assessing ferricyanide reduction in intact red blood cells treated with various flavonoids.
  • Investigating the direct reduction of ferricyanide by flavonoids.

Related Experiment Videos

  • Evaluating the role of intracellular flavonoid concentration and the effect of sulphydryl reagents like parachloromercuribenzenesulfonic acid (pCMBS).

    • Main Results:

    • Quercetin and myricetin significantly stimulated extracellular ferricyanide reduction, unlike other tested flavonoids.
    • Evidence suggests quercetin and myricetin act as intracellular electron donors, correlating with enzyme activity and declining upon ferricyanide exposure.
    • The observed reduction was sensitive to pCMBS, indicating the involvement of a specific trans-plasma membrane oxidoreductase.

    Conclusions:

    • Myricetin and quercetin function as intracellular substrates for a pCMBS-sensitive trans-plasma membrane oxidoreductase in red blood cells.
    • This interaction represents a novel mechanism by which these flavonoids may confer protection against oxidative stress.