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Knowledge-based system for method development of chiral separations with capillary electrophoresis using
N Matthijs1, C Perrin, M Maftouh
1Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit, Laarbeeklaan 103, B-1090 Brussels, Belgium.
Journal of Pharmaceutical and Biomedical Analysis
|January 5, 2002
Summary
Developing chiral separations is challenging. A new knowledge-based system (KBS) aids analysts in rapidly determining experimental parameters for baseline enantiomer separation, specifically detailing capillary electrophoresis (CE) methods using sulfated cyclodextrins.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Chiral separations are complex, requiring significant expertise and experimental trials.
- Developing methods for baseline enantiomer separation is a common challenge in analytical chemistry.
Purpose of the Study:
- To develop a knowledge-based system (KBS) to expedite method development for chiral separations.
- To provide a tool for rapid determination of experimental parameters for enantiomer separation.
Main Methods:
- A KBS was constructed using in-house laboratory knowledge and literature data.
- Rules were defined for five different separation techniques.
- The capillary electrophoresis (CE) section focuses on highly-sulfated cyclodextrins as chiral selectors.
Main Results:
- A structured knowledge representation was developed for the KBS.
- The KBS was implemented using Toolbook software.
- A specific strategy for CE using sulfated cyclodextrins was defined.
Conclusions:
- The developed KBS simplifies and accelerates chiral separation method development.
- The system offers a structured approach to parameter determination, particularly for CE.
- This knowledge-based approach aids analysts in achieving baseline enantiomer separation efficiently.