Knowledge-based system for method development of chiral separations with capillary electrophoresis using highly-sulphated cyclodextrins
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Updated: Oct 3, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
N Matthijs1, C Perrin, M Maftouh
1Department of Pharmaceutical and Biomedical Analysis, Pharmaceutical Institute, Vrije Universiteit, Laarbeeklaan 103, B-1090 Brussels, Belgium.
Abstract:
Method development for chiral separations is not easy because it requires experience and many experimental possibilities can be chosen. In order to help the analyst, a knowledge-based system (KBS) for the rapid determination of experimental parameters, which allow a baseline separation of enantiomers, has been developed. On the basis of own laboratory knowledge, completed with literature data, rules were defined and a KBS was built. Five different techniques are considered in this KBS. This paper describes the capillary electrophoresis (CE) section, in which a strategy has been defined based on the use of highly-sulfated cyclodextrins as chiral selectors. A structured representation of the knowledge and its implementation in Toolbook software is presented.
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