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Related Experiment Videos

Germinal vesicle transfer between fresh and cryopreserved immature mouse oocytes.

Federica Moffa1, Francesca Comoglio, Lewis C Krey

  • 1Program for In vitro Fertilization, Reproductive Surgery and Infertility, NYU School of Medicine, New York, USA. femoffa@hotmail.com

Human Reproduction (Oxford, England)
|January 5, 2002
PubMed
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Cryopreserved immature oocytes can be used for germinal vesicle (GV) transfer, yielding chromosomally normal oocytes capable of reaching metaphase II. This technique offers potential applications in research and clinical settings.

Area of Science:

  • Reproductive Biology
  • Cryobiology
  • Oocyte Maturation

Background:

  • Assessing maturational competence and chromosomal patterns of mouse oocytes reconstructed via germinal vesicle (GV) transfer.
  • Utilizing nuclear and/or cytoplasmic components from cryopreserved GV-stage oocytes for reconstruction.

Purpose of the Study:

  • To evaluate the viability and chromosomal normality of oocytes reconstructed using cryopreserved components.
  • To determine the potential of germinal vesicle transfer with cryopreserved oocytes for research and clinical applications.

Main Methods:

  • Reconstruction of 657 GV oocytes into four groups (fresh/thawed nuclear/cytoplasmic components) using micromanipulation and electrofusion.
  • In vitro culture of reconstructed oocytes to the metaphase II stage.

Related Experiment Videos

  • Assessment of survival, maturation rates, and chromosomal analysis.

    • Main Results:

    • No significant differences in survival or progression to metaphase II among the four reconstruction groups.
    • A significantly higher maturation rate observed in the thawed GV-thawed cytoplast group compared to controls (P < 0.01).
    • 88% of reconstructed oocytes exhibited the correct chromosomal number (20 chromosomes).

    Conclusions:

    • Nuclear and cytoplasmic components from cryopreserved immature oocytes are suitable for GV transfer.
    • Reconstructed oocytes can achieve metaphase II and possess normal chromosomal patterns.
    • Cryostored immature oocytes offer a viable source for GV transfer applications in research and clinics.