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[A study on beta-lactamase activity of biofilm Escherichia coli]
Objective:
To investigate the mechanism of drug resistance of biofilm escherichia coli.
Methods:
The model of escherichia coli biofilm was established with the flat-board method. And the biofilm was confirmed by scanning electron microscopy. The beta-lactamase activities were quantitated, in escherichia coli, biofilm escherichia coli, biofilm escherichia coli induced by impenem or cefoxitin.
Results:
The beta-lactamase activity of biofilm escherichia coli was 2.16 times as much as that of escherichia coli planctonically, and the beta-lactamase activities of biofilm escherichia coli induceded by impenem or cefoxitin were 1.30 and 1.05 times as much as those of biofilm escherichia coli, respectively.
Conclusion:
The drug resistance to antibiotics of biofilm escherichia coli was related to the production of beta-lactamase.