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[Expression of cytokines in acute heart transplantation rejection]
1Department of Cardiac Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Objective:
To observe the expression and changes of local cytokines network in rat heart transplantation so as to determine the role of cytokines in acute rejection of rat heart transplantation.
Methods:
The Wistar rats (receptor) were divided into four groups: group A(control, n = 12), group B(IL-2 Mab treated, n = 12), group C [Cyclosporine A (CsA) treated, n = 12] and group D(IL-2Mab + CsA treated, n = 12). Hearts from SD rats were transplanted into a cervical location in Wistar. The local expression of IL-1 beta, IL-2, CD25, IL-4, IL-5, IL-6, IL-10, TNF alpha, IFN gamma was examined at 1, 3, 5, 7, 9, 11, 14 days after operation by reverse transcription polymerase chain reaction (RT-PCR).
Results:
Allografts survival time was (8.3 +/- 1.7) days in group A, (29.2 +/- 7.1) days in group B, (26.4 +/- 5.7) days in group C, (55.0 +/- 10.6) days in group D. The expression of IL-1 beta, IL-4, IL-10, IFN gamma was up-regulated, and the expression of IL-2, CD25, IL-5, IL-6, TNF alpha was significantly inhibited in group A. The expression of IL-1 beta, IL-5, IL-6, IL-10, IFN gamma was up-regulated, and the expression of IL-2, IL-4, TNF alpha was significantly down-regulated in group B. The expression of IL-1 beta, IL-2, CD25, IL-5, TNF alpha, IFN gamma was up-regulated, and the expression of IL-4, IL-6, Il-10 was significantly down-regulated in group C. The expression of IL-4, IL-5, IL-6, IL-10 was up-regulated, and the expression of IL-1 beta, IL-2, CD25, IFN gamma, TNF alpha was significantly down-regulated in group D.
Conclusions:
Cytokines play an important role in the development of acute transplantational rejection. Different cytokines play different roles in different local environments; the main principle possibly is immune deviation.