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[Changes in plasma free amino acid concentrations in rats during sepsis]
1Burns Institute, 304th Hospital, People's Liberation Army, Beijing 100037, China.
Objective:
To explore the relationship between plasma free amino acid concentrations and skeletal muscle proteolysis in rats during sepsis.
Methods:
An animal model of sepsis in rats was established by administering endotoxin into the peritoneal cavity. The plasma concentrations of free amino acid, ALT, AST, cortisol, TNF-alpha and IL-6 were determined. The rats were randomly divided into normal control, 2, 6, 12 and 24 h groups after administration of endotoxin(8 rats for each group).
Results:
The total plasma free amino acids concentrations fluctuate within normal range within 24 h after administration of endotoxin. A marked decrease in BCAA (branch-chainamino acid) was observed at 2, 6 and 12 h after administration of endotoxin, but aromatic amino acids (AAA) increased in varying degrees. BCAA/AAA was lower at 2 h and 6 h as compared with that of the normal control, while Phe/Tyr increased obviously at every time point. Lys, Sar, Cys and P-Ser showed no significant changes within 24 h after the administration of endotoxin. Thr, Glu, Orn, His, 3-MH and Ala increased during sepsis, and other amino acids more or less decreased. Plasma concentrations of ALT, AST, cortisol, TNF-alpha and IL-6 obviously increased within 24 h after the administration of endotoxin(P < 0.01). Cortisol peaked at 6 h and IL-6 peaked at 12 h, while TNF-alpha at 2 h.
Conclusion:
The results suggested that metabolic disorder of plasma free amino acids during sepsis is due to enhancement of skeletal muscle proteolysis and liver overloading.