Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Aanalysis of resistance and its transferability in microflora from primary meat production].

M Malíková, Z Matyás

    Veterinarni Medicina
    |October 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Carriage of Neisseria meningitidis in newly enlisted young military professionals during the COVID-19 pandemic

    Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2023
    Same author

    Ectodermal dysplasia: important role of complex dental care in its interdisciplinary management.

    European journal of paediatric dentistry·2022
    Same author

    Steroid diagnostics of 21st century in the light of their new roles and analytical tools.

    Physiological research·2020
    Same author

    Molecular genetic analysis in 14 Czech Kabuki syndrome patients is confirming the utility of phenotypic scoring.

    Clinical genetics·2016
    Same author

    The evaluation of a novel technique to treat saphenous vein incompetence: preclinical animal study to examine safety and efficacy of a new vein occlusion device.

    Phlebology·2012
    Same author

    Double heterozygosity with mutations involving both the GJB2 and GJB6 genes is a possible, but very rare, cause of congenital deafness in the Czech population.

    Annals of human genetics·2005

    Most meat-associated Gram-negative strains exhibit resistance, with many showing multidrug resistance. R plasmids frequently transfer, primarily conferring tetracycline and ampicillin resistance, impacting food safety and public health.

    Area of Science:

    • Microbiology
    • Food Science
    • Genetics

    Background:

    • Antibiotic resistance in bacteria is a growing concern, particularly in food production environments.
    • Understanding the prevalence and transferability of resistance genes is crucial for public health.
    • Gram-negative bacteria in meat production are potential reservoirs for antibiotic resistance.

    Purpose of the Study:

    • To investigate the resistance spectra and R plasmid transferability in Gram-negative strains from meat production.
    • To identify common resistance patterns and the frequency of multidrug resistance.
    • To assess the efficiency of R plasmid transfer to a laboratory strain.

    Main Methods:

    • Isolation and characterization of Gram-negative strains from meat production using Bohus's method (1974).

    Related Experiment Videos

  • Determination of resistance spectra through phenotypic testing.
  • Conjugation experiments to assess R plasmid transferability to an Escherichia coli recipient strain.

    • Main Results:

    • 93% of isolated strains were resistant, with 65% exhibiting multidrug resistance.
    • 35% of resistant strains acted as R plasmid donors.
    • Tetracycline (Tc), streptomycin-tetracycline-sulfonamide (SmTcSu), and tetracycline-ampicillin (TcAm) were the most frequent resistance spectra.
    • Transfer of 2-6 plasmid types occurred, with Tc and ampicillin (Am) determinants being prevalent in R plasmids.

    Conclusions:

    • Gram-negative bacteria in meat production harbor significant antibiotic resistance, including multidrug resistance.
    • R plasmids are transferable, with Tc and Am resistance determinants being commonly transferred.
    • These findings highlight the potential for antibiotic resistance dissemination from food production to the environment and human populations.