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Published on: August 17, 2009
Isolation and characterization of isolectins from Talisia esculenta seeds
M G Freire1, O L Machado, M B Smolka
1Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (Unicamp), SP, Brazil.
Abstract:
Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (Mr 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates.
