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Updated: Jul 17, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
[Polymorphism of hordeins in Ethiopian barley]
1Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia. Pomortsev@vigg.ru
Abstract:
Using starch gel electrophoresis, polymorphism of hordein-encoding loci Hrd A, Hrd B, and Hrd F was studied in 147 accessions of local Ethiopian barley varieties. Loci Hrd A, Hrd B, and Hrd F were shown to have 26, 36, and 4 alleles, respectively. The allele frequencies in the collection examined varied from 0.17 to 45.72%. For loci Hrd A and Hrd B, families of blocks of hordein components were found. Based on the allele frequencies and their combinations at loci Hrd A and Hrd B as well as the numbers of families of component blocks in hordeins A and B, we identified genotypes that could be considered as the most ancient in Ethiopia. A catalog of hordein variant encoded by these loci was created. The list of hordein genetic formulas for the studied accessions is presented.
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