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Dual-substrate utilization by Bordetella pertussis
R Neeleman1, M Joerink, C Beuvery
1National Institute of Public Health and the Environment, Laboratory for Product and Process Development, Bilthoven, The Netherlands. Ronald.Neeleman@rivm.nl
Applied Microbiology and Biotechnology
|January 5, 2002
Summary
A new model improves Bordetella pertussis cultivation by quantitatively describing substrate utilization. This essential kinetics model enhances monitoring and control strategies for the bacterium.
Area of Science:
- Microbiology
- Biotechnology
- Bioprocess Engineering
Background:
- Bordetella pertussis cultivation requires improved methods for enhanced monitoring and control.
- Current modeling approaches are insufficient for accurately describing Bordetella pertussis growth kinetics.
- Understanding substrate utilization is crucial for optimizing bacterial growth.
Purpose of the Study:
- To develop a quantitative model for Bordetella pertussis substrate utilization.
- To improve the efficiency and accuracy of Bordetella pertussis cultivation.
- To provide a foundation for advanced monitoring and control techniques.
Main Methods:
- Development of a novel model combining essential and enhanced kinetics.
- Utilizing experimental observations for model parameterization.
- Implementing a step-wise procedure for model parameter fitting.
- Validation through two experimental cultivations.
Main Results:
- A quantitative model accurately describing Bordetella pertussis growth was successfully developed.
- The model integrates essential and enhanced kinetic parameters.
- Step-wise parameter fitting proved effective for model calibration.
- Experimental cultivations confirmed the model's predictive accuracy.
Conclusions:
- The developed model offers a significant improvement for Bordetella pertussis cultivation.
- This quantitative description facilitates enhanced monitoring and control strategies.
- The model provides a robust framework for bioprocess optimization.