Electrogenic properties of the Na+,K+-ATPase probed by presteady state and relaxation studies
E Bamberg1, R J Clarke, K Fendler
1Max-Planck-Institut für Biophysik, Frankfurt am Main, Germany. ernst.bamberg@mpibp-frankfurt.mpg.de
Abstract:
Electrical measurements on planar lipid bilayers, patch/voltage clamp experiments, and spectroscopic investigations involving a potential sensitive dye are reviewed. These experiments were performed to analyze the kinetics of charge translocation of the Na+,K+-ATPase. High time resolution was achieved by applying caged ATP, voltage-jump, and stopped-flow techniques, respectively. Kinetic parameters and the electrogenicity of the relevant transitions in the Na+,K+-ATPase reaction cycle are discussed.
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