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Isolation and identification of mycoplasma agalactiae subsp. bovis from arthritic cattle in Iowa and Nebraska
Journal of Clinical Microbiology
|September 1, 1975
Abstract:
Two strains of Mycoplasma were isolated from synovial fluids of arthritic feeder cattle and were identified as Mycoplasma agalactiae subsp. bovis by growth inhibition and fluorescent antibody tests. The strains (Iowa 1136 and Nebraska 2) could not be distinguished from known strains (Donetta and California 01) by immunoelectrophoresis or by agar gel precipitation.
Insights
Two Mycoplasma strains were isolated from arthritic cattle and identified as Mycoplasma agalactiae subsp. bovis. These novel strains were indistinguishable from known Mycoplasma agalactiae subsp. bovis strains using advanced laboratory techniques.
Area of Science:
- Veterinary Microbiology
- Bovine Pathology
Background:
- Arthritis in feeder cattle can be caused by bacterial infections.
- Mycoplasma species are known pathogens in cattle, affecting various systems.
Purpose of the Study:
- To identify the Mycoplasma species responsible for arthritis in feeder cattle.
- To characterize newly isolated Mycoplasma strains from affected cattle.
Main Methods:
- Isolation of bacteria from synovial fluid samples.
- Identification using growth inhibition and fluorescent antibody tests.
- Serological comparison with known strains via immunoelectrophoresis and agar gel precipitation.
Main Results:
- Two Mycoplasma strains, Iowa 1136 and Nebraska 2, were successfully isolated.
- Both isolates were identified as Mycoplasma agalactiae subsp. bovis.
- No significant antigenic differences were detected between the new isolates and established strains (Donetta, California 01).
Conclusions:
- Mycoplasma agalactiae subsp. bovis is a causative agent of arthritis in feeder cattle.
- The isolated strains are antigenically similar to previously identified strains, suggesting potential epidemiological links or strain conservation.