[Factors affecting transformation of Agrobacterium tumefaciens and their application on cereals]

Agrobacterium tumefaciens mediated genetic transformation is the method most widely used in plant transformation. How to improve its transformation efficiency and extend its host range to include most cereals is what people concern about. There are many factors that influence the transformation efficiency, including the wounding response of plants, attachment of bacteria, induction of virulent gene, DNA repair and replication activity of plant cells, state of explant, etc. Recent research has proved that cerelas can be transformed effectively by A. tumefaciens under suitable conditions. This paper reviewed the recent progress in the two aspects.