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Microchromatography of hemoglobins. II. A simplified procedure for the determination of hemoglobin A2

T H Huisman, W A Schroeder, A N Brodie

The Journal of Laboratory and Clinical Medicine

|October 1, 1975

Abstract:

A modification of the microchromatographic procedure for Hb-A2 which utilizes DEAE-cellulose (Reference 1) allows the quantitation of Hb-A2 without interference from any Hb-S in the sample. Elution of Hb-A2 with a glycine-KCN developer is much less sensitive to minor change in pH of the developer, but is greatly dependent on the pH of the ion exchanger.

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