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The catalytic mechanism of nucleoside diphosphate kinases
1Institut de Biochimie et Génétique Cellulaires, UMR 5095 University of Bordeaux-2 and CNRS, France. ioan.lascu@ibgc.u-bordeaux2.fr
Journal of Bioenergetics and Biomembranes
|January 5, 2002
Abstract:
Nucleoside diphosphate kinases catalyze the reversible transfer of the gamma phosphate of nucleoside triphosphates to nucleoside diphosphates. This minireview presents recent advances in understanding the reaction mechanism using steady-state and fast kinetic studies, X-ray crystallography, and site-directed mutagenesis. We also briefly discuss the physiological relevance of in vitro studies.