The distribution of non-synaptic intercellular junctions during neurone differentiation in the developing spinal cord...

The distribution of intercellular junctions, other than synapses and their precursors, has been described in the developing spinal cord of Xenopus laevis between the neurula and free swimming tadpole stages. At the neurocoel, ventricular cells are joined in the apical contact zone by a sequence of junctions which usually has one or more intermediate junctions but also includes close appositions, gap junctions and desmosomes. This apical complex is more diverse than that reported in other vertebrate embryos and between ependymal cells in the adult central nervous system. Gap junctions are also found between ventricular cells and their processes near the external cord surface. However, no other special junctions occur in this location under the basement lamella which surrounds the cord. Punctate intermediate junctions are generally distributed between undifferentiated and differentiating cells and their processes but were not found in neuropil after stage 28. These results are discussed in relation to cell movements during neural differentiation, possible effects on the freedom of movement of ions and molecules through extracellular pathways in the emryo, and possible intercytoplasmic pathways via gap junctions which may be responsible for the physiologically observed electrical coupling between neural tube cells.