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Differentiating vector-derived mRNA from contaminating DNA templates by inverse RT-PCR
S Flindt1, M Meier-Noorden, T Hinz
1Paul Ehrlich Institute, Langen, Germany.
Biotechniques
|January 5, 2002
Summary
This study introduces a novel method for detecting doxycycline-induced mRNA expression using inverse RT-PCR. The technique selectively amplifies transcripts from integrated vector genomes, eliminating the need for DNase treatment.
Area of Science:
- Molecular Biology
- Gene Expression Analysis
- Retroviral Vector Technology