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Homemade Site Directed Mutagenesis of Whole Plasmids
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Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

Two-stage polymerase chain reaction protocol allowing introduction of multiple mutations, deletions, and insertions,

Wenyan Wang1, Bruce A Malcolm

  • 1Department of Structural Chemistry, Schering-Plough Research Institute, Kenilworth, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 5, 2002
PubMed
Abstract

No abstract available in PubMed .

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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