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[Detection of -5/5q- chromosome abnormality in myelodysplastic syndromes by interphase fluorescence in situ
1First Affiliated Hospital, Soochow University, Jiangsu Institute of Hematology, Suzhou 215006, China.
Objective:
To evaluate the value of interphase fluorescence in situ hybridization(FISH) in the detection of acquired complete and partial deletion of chromosome 5(-5/5q-) in myelodysplastic syndromes (MDS).
Methods:
Marrow cells from 48 MDS patients and 10 normal controls were studied by interphase FISH using SpectrumRed directly labelled DNA specific probe for 5q31, 200 to 300 cells were scored for each case and cells with a red hybridization signal > 6% were defined as -5/5q- positive.
Results:
In 48 MDS patients, 13 were positive for interphase FISH, of whom, 7 were positive and 6 were negative for conventional cytogenetics (CC). The difference in the percentage of positive cells detected by the two methods was statistically significant (P < 0.05).
Conclusion:
Interphase FISH is more sensitive than CC for the detection of -5/5q- in MDS.
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