Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Interaction of E. coli cells with ascorbic acid and sodium nitrite studied by ESR method].

T T Zhumbaeva1, L M Baĭder, L A Volodina

  • 1Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 117977 Russia.

Prikladnaia Biokhimiia I Mikrobiologiia
|January 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Complexes of nitric oxide with hemoglobin and paramagnetic metalloenzymes in the brain and blood of mammals after intermittent hypoxia].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2016
Same author

[Copper-induced change in the ESR signal of hemoglobin nitrosyl complexes in wound by the action of copper nanoparticles].

Biofizika·2014
Same author

[The influence of physical-chemical characteristics of surface modified copper nanoparticles on E. coli cell population growth suppression and on electrostatic properties of their membranes].

Biofizika·2013
Same author

[The use of hydroxamic acids and sodium nitrate to enhance the antitumor effect of cyclophosphamide].

Voprosy onkologii·2013
Same author

[Atorvastatin and oxidative stress in coronary heart disease with obesity].

Klinicheskaia meditsina·2013
Same author

[Experimental hemorrhagic stroke: the effect of the peptide preparation cortexin in the formation of Hb-NO-complexes and other blood paramagnetic centers].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2012
Same journal

[Fluorescence polarization immunoassay of ractopamine].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same journal

[Genetic system for maintaining the mitochondrial human genome in yeast Yarrowia lipolytica].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same journal

[Formation of protodioscin and deltoside isomers in suspension cultures of Nepal yam (Dioscorea deltoidea Wall.) cells].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same journal

[New strains of basidiomycetes that produce bioethanol from lignocellulose biomass].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same journal

[Oil degradation by basidiomycetes in soil and peat at low temperatures].

Prikladnaia biokhimiia i mikrobiologiia·2018
Same journal

[Degradation of fluorene and fluoranthene by the basidiomycete Pleurotus ostreatus].

Prikladnaia biokhimiia i mikrobiologiia·2018
See all related articles

Ascorbic acid triggers nitric oxide production in E. coli, detected via EPR spectroscopy. This vitamin also aids cell energy supply during low oxygen conditions.

Area of Science:

  • Microbiology
  • Biochemistry
  • Cellular Metabolism

Background:

  • Ascorbic acid (vitamin C) is a known regulator of cellular metabolism.
  • Nitric oxide (NO) plays crucial roles in various biological processes.
  • Escherichia coli (E. coli) is a model organism for studying bacterial metabolism.

Purpose of the Study:

  • To investigate the effect of ascorbic acid on nitric oxide production in E. coli.
  • To explore the mechanism of NO generation and its detection.
  • To understand ascorbic acid's role in cellular energy supply under hypoxic conditions.

Main Methods:

  • Electron Paramagnetic Resonance (EPR) spectroscopy was used to detect Fe-S-NO centers.
  • E. coli cultures were incubated with ascorbic acid under both anaerobic and aerobic conditions.

Related Experiment Videos

  • Sodium nitrite formation was measured under aerobic conditions.

    • Main Results:

    • EPR spectroscopy confirmed the generation of nitrosyl-iron-sulfur centers (Fe-S-NO) in E. coli under anaerobic conditions with ascorbic acid.
    • Sodium nitrite was detected in E. coli cultures incubated with ascorbic acid under aerobic conditions.
    • Ascorbic acid appears to support cellular energy metabolism during hypoxia.

    Conclusions:

    • Ascorbic acid induces nitric oxide production in E. coli.
    • The Fe-S-NO signal indicates a specific metabolic pathway activated by ascorbic acid.
    • Ascorbic acid may play a protective role in cellular energy homeostasis under oxygen-deficient environments.