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Updated: Oct 3, 2026

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
Published on: June 9, 2017
Dissecting receptor-G protein specificity using G alpha chimeras
Theresa M Cabrera-Vera1, Tarita O Thomas, Jurgen Vanhauwe
1Department of Molecular Pharmacology and Biochemistry, Institute for Neuroscience, Northwestern University, Chicago, Illinois 60611, USA.
Abstract:
In conclusion, by taking advantage of the overall sequence homology and structural similarity of G alpha subunits, functional chimeric G alpha subunits can be generated and used as tools for the identification of sequence-specific factors that mediate receptor: G protein specificity. The [35S]GTP gamma S binding assay and the affinity shift activity assay are two sensitive biochemical approaches that can be used to assess receptor: G protein coupling in vitro. These in vitro assays limit confounding influences from cellular proteins and allow for the strict control of receptor: G protein ratios.
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