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Updated: Jul 28, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
Innate immune reactions stimulated by a lipopolysaccharide-like component of the alga Prototheca (strain 289)
J C Bedick1, A Shnyra, D W Stanley
1Insect Biochemical Physiology Laboratory, 311 Plant Industry Building, University of Nebraska, Lincoln, Nebraska, NE 68583-0816, USA. dstanley1@unl.edu
Abstract:
We report on the influence of an LPS-like molecule (aLPS) from the pathogenic alga, Prototheca (strain 289) on insect and murine innate immune reactions. Insect innate reactions to infection include nodule formation, a process of entrapping bacterial cells in aggregates of hemocytes. We recorded eicosanoid-dependent, dose-related nodulation reactions to aLPS in hornworms (Manduca sexta). The insect reaction was attenuated by pre-incubating the aLPS with polymyxin-B. Conversely, the murine macrophages reacted to challenge with Escherichia coli LPS by secreting cytokines, but did not react to aLPS. We infer that, while highly conserved with respect to intracellular mechanisms of interaction, insect and mammalian immune surveillance systems differ in recognition of LPS molecular types.
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