Threonine requirements of different broiler genotypes

The objectives of this work were to study the responses of one Leghorn and two broiler stocks and sexes to different levels of Thr and to estimate their requirements for this amino acid. All experiments were conducted with birds from 1 to 18 d of age. Body weight gain (BWG) and feed consumption were measured on the eighteenth day, and feed conversion ratio (FCR) was calculated. At Day 18, three birds per replicate were killed, and liver and fat pads were collected and weighed. The data were analyzed by the general linear model and nonlinear model procedures of SAS software, and the broken-line linear model was used to estimate Thr requirements of chicks. Experiment 1 had a 5 x 3 factorial design with five levels of Thr (0.63, 0.70, 0.77, 0.84, and 0.91% of the diet), two broiler genotypes (Arbor Acres Classic and High Yield), and one Leghorn genotype (Hy-line W-36), with three replicate pens of eight male birds each. The basal diet was composed of corn, peanut meal (PNM), poultry by-product meal, poultry fat, DL-Met, L-Lys, and L-Ile (23% CP and 3.2 kcal/g of ME). The Leghorn chicks did not respond to Thr supplementation, indicating their requirement was < or = 0.63%. High Yield birds had better BWG and FCR than Classic. The BWG and FCR were reduced more in Classic than High Yield strains by the lowest Thr level. The percentage of liver was higher in the Classic than High Yield strain broilers with Thr supplementation. The second experiment (2 x 2 x 2 factorial design) was conducted to evaluate the effects of two levels of Thr (0.63 and 0.90%), two levels of Ile (0.72 and 0.90%), and two broiler strains (as Experiment 1). There were no responses to Ile for any parameters measured. Experiment 3 (6 x 2 factorial design, corn, PNM-based basal diet) had six Thr levels (0.63, 0.67, 0.71, 0.75, 0.79, and 0.83%) and two broiler strains (as Experiment 1), with four replicate pens of eight male birds each. The High Yield strain broilers grew significantly better at the lowest Thr level, but performance was similar at the higher Thr levels (significant interaction, P = 0.018). FCR was affected by Thr level but not by strain. The Thr requirement of the Classic strain broilers was 0.69 +/- 0.01% for BWG and 0.68 +/- 0.01% for FCR. The Thr requirement of the High Yield strain broilers was 0.68 +/- 0.01% for BWG and 0.69 +/- 0.01% for FCR. The Thr requirements of male versus female High Yield strain broilers were evaluated in a 6 x 2 factorial design, with six Thr levels (Experiment 4), with four replicate pens of eight birds each. Males and females had the same performance and carcass composition results (P > 0.05), except for percentage abdominal fat pads. The Thr requirement was 0.71 +/- 0.01% for BWG and 0.71 +/- 0.01% for FCR for the males and 0.72 +/- 0.008% for BWG and 0.71 +/- 0.001% for FCR for females. Excellent performance was observed from the corn- and PNM-based diet supplemented to contain 0.72% Thr. The Thr requirements of High Yield and Classic broilers (males and females) were similar and greater than for the Leghorn strain studied.