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Cryptosporidium parvum mixed genotypes detected by PCR-restriction fragment length polymorphism analysis
Carrie Reed1, Gregory D Sturbaum, Paul J Hoover
1Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona 85721, USA.
Applied and Environmental Microbiology
|January 5, 2002
Abstract:
Combinations of 10 Cryptosporidium parvum oocysts, with various ratios of genotype I to genotype II, were isolated and subjected to PCR-restriction fragment length polymorphism analysis. Amplification of both genotypes in these samples ranged from 31 to 74% and yielded no information about the genotype proportions. In addition, since both genotypes were not always detected, amplification of a single genotype is not conclusive evidence that the sample contains only a single genotype.