Related Experiment Video
Updated: Oct 3, 2026
Soybean Hairy Root Transformation for the Analysis of Gene Function
Published on: May 5, 2023
Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7
1Agriculture and Agri-food Canada, Greenhouse and Processing Crop Research Centre, 2585 Highway 20 E., Harrow, Ontario, N0R 1G0 Canada. wengc@em.agr.ca
Abstract:
A linkage map was constructed for two Phytophthora sojae Kauf. +Gerd. root rot resistance genes, Rps1a and Rps7, in soybean (Glycine max (L.) Merr.) using microsatellite or simple sequence repeat (SSR) markers. An F2 population consisting of 81 individuals derived from a cross between OX281, which carries Rps7, and Mukden, which carries Rps1a, was used as the mapping population. A linkage map consisting of 10 SSR markers was first constructed using the computer software MapMaker/EXP 3.0. Rps1a and Rps7 were then placed at two different loci in the same linkage group with LOD scores of 2.88 and 9.16, respectively. Rps1a and Rps7 were linked at a distance of 13.8 cM. Rps1a was flanked by Satt159 (0.7 cM) and Satt009 (3.2 cM). Rps7 was flanked by Satt009 (10.6 cM) and Satt125 (29.1 cM).
