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A compendium of gene expression in normal human tissues
L L Hsiao1, F Dangond, T Yoshida
1Renal Division, Department of Medicine, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston 02115, USA.
Physiological Genomics
|January 5, 2002
Summary
This study generated a gene expression atlas for normal human tissues, identifying 451 housekeeping genes. This resource reveals tissue-specific gene signatures, aiding organ system biology and pathophysiology research.
Area of Science:
- Genomics
- Molecular Biology
- Human Physiology
Background:
- Understanding normal human tissue gene expression is crucial for defining organ system biology.
- A comprehensive reference is needed to interpret gene expression patterns in health and disease.
Purpose of the Study:
- To create a gene expression compendium for normal human tissues.
- To establish a reference for basic organ systems biology.
- To identify housekeeping and tissue-selective genes.
Main Methods:
- Oligonucleotide microarrays were used to analyze 59 samples from 19 distinct human tissue types.
- Gene expression profiles were analyzed across all samples.
- Statistical methods were employed to identify housekeeping and tissue-selective genes.
Main Results:
- A compendium of gene expression in normal human tissues was created.
- 451 genes were identified as housekeeping genes, expressed in all analyzed tissues.
- Significant variations in housekeeping gene expression and tissue-specific gene signatures were observed, reflecting fundamental biochemical differences.
Conclusions:
- The study provides a valuable reference for organ systems biology and pathophysiology.
- Housekeeping genes, despite ubiquitous expression, show significant inter-tissue variation.
- Tissue-selective genes define key biological processes characterizing each organ, highlighting similarities and differences across human tissues and individuals.
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