Related Experiment Videos
[Microsatellite polymorphisms at the TNF locus in Hubei Han population]
1Gastroenterology Department, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, 310014 P.R.China. feibaoying6924@163.net
Objective:
To investigate the distribution of tumor necrosis factor(TNF) microsatellite polymorphisms in Chinese.
Methods:
DNA was extracted from 164 unrelated healthy individuals' EDTA-blood. TNF microsatellite alleles were typed using PCR technique followed by high voltage denaturing PAGE with silver staining at the same time the PCR products were cloned and sequenced.
Results:
Thirteen alleles and forty kinds of genotypes were detected at the TNFa locus; two alleles and three kinds of genotypes at the TNFa locus. The polymorphism information contents (PIC) were 0.80 and 0.28 respectively no deviation from Hardy-Weinberg equilibrium was observed. Statistical analysis showed that the distribution of TNFa allele frequencies in Chinese Han population was significantly different from that in European Caucasian or in Japanese P<0.01. The result of sequencing revealed that the copy number of dinucleiotide repeats within the same TNF allele was not consistent with that in the reports from western countries.
Conclusion:
There exists ethnic difference in the distribution of TNFa allele. Further investigations will be necessary to define more accurately and clearly the TNFa alleles.