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[Chemotactic response of polymorphonuclear neutrophil to FMLP and IL-8 in rapidly progressive periodontitis patients]
Objective:
It has been reported that 48%-85% of rapidly progressive periodontitis (RPP) are associated with a defect in polymorphonuclear neutrophil (PMN) chemotactic function. However, some reports failed to detect any defect in PMN chemotaxis in RPP patients. Further, the influence of race on neutrophil function has been suggested. The purpose of the present study was to determine the PMN chemotactic function in Chinese RPP patients.
Methods:
Neutrophils were obtained from heparinized peripheral blood of 16 RPP and 14 periodontally healthy control subjects matched for age and sex. Cells were isolated by Percoll discontinuous density gradient centrifugation. Chemotaxis was evaluated using modified Boyden chamber. (N-formyl-methionyl-leucyl-phenylalanine, FMLP) (10(-8) mol/L) and IL-8 (100 micrograms/L) which is a more specific neutrophil chemokines were used in this study. The results were determined by staining the filters followed by visual counting under microscopy at 600 x. Data was analyzed using the Students' t test and the value of RPP was compared with that of control subjects(> mean +/- 2s or < mean +/- 2s).
Results And Conclusion:
The results showed all RPP patients had normal PMN chemotactic behavior.
Insights
Rapidly progressive periodontitis (RPP) patients, particularly those of Chinese ethnicity, exhibited normal polymorphonuclear neutrophil (PMN) chemotactic function. This study found no defects in PMN function in the RPP group.
Area of Science:
- Periodontology
- Immunology
- Cell Biology
Context:
- Rapidly progressive periodontitis (RPP) is a severe form of gum disease.
- Previous studies suggest a link between RPP and impaired neutrophil function, but findings are inconsistent.
- The role of ethnicity in neutrophil function in RPP remains unclear.
Purpose:
- To investigate the chemotactic function of polymorphonuclear neutrophils (PMNs) in Chinese patients with RPP.
- To compare PMN chemotaxis in RPP patients with periodontally healthy controls.
Summary:
- Neutrophils were isolated from 16 RPP patients and 14 healthy controls.
- Chemotaxis was assessed using N-formyl-methionyl-leucyl-phenylalanine (FMLP) and IL-8.
- All RPP patients demonstrated normal PMN chemotactic behavior.
Impact:
- Suggests that PMN chemotactic dysfunction may not be a universal feature of RPP, especially in the studied Chinese population.
- Highlights the need for further research into potential ethnic variations in neutrophil function related to periodontitis.
- Contributes to a better understanding of the pathogenesis of RPP.
