Related Experiment Video
Updated: Oct 3, 2026
Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
Published on: May 1, 2014
[The effect of retinoic acid on crown morphogenesis of mouse tooth germs in vitro]
Objective:
To study the effects of retinoic acid on crown morphogenesis of early tooth germs.
Methods:
Mouse tooth organ culture model was used and the functions of different retinoic acid concentration on the first mandibular molar germs of 14-day old mouse embryo were examined.
Results:
The first mandibular molar germ of 14-day old mouse was at the bud stage. After 6 days culture, tooth germs in culture medium (not containing retinoic acid) developed from bud stage to bell stage and formed normal cusps. The morphogenesis of tooth germs in medium containing 1 x 10(-7) mol/L retinoic acid was similar to molar germs in the medium without retinoic acid. When the concentration of retinoic acid increased to 1 x 10(-6) mol/L, the mouse tooth germs still developed from bud stage to bell stage, but normal cusps was not formed.
Conclusion:
The results suggest that high concentration of retinoic acid alter crown morphogenesis of tooth germs.
