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Updated: Oct 3, 2026
Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
Published on: May 4, 2018
[1,25(OH)2 vitamin D3 induces IL-1 alpha mRNA expression from osteoblastic-like and bone marrow co-culture cells]
Objective:
The purpose of this research is to investigate the relationship between 1,25(OH)2 Vitamin D3[1,25(OH)2D3] and Interleukin-1 (IL-1) in regulating osteoclast formation. The result may enrich our knowledge about biological mechanisms of periodontal tissue remodeling during orthodontic tooth movement.
Methods:
We found IL-1 alpha mRNA expression in bone marrow and osteoblastic cell co-cultures by semi-quantitative RT-PCR.
Results:
1,25(OH)2D3 increased IL-1 alpha mRNA expression from this co-culture cells in a dose dependent manner, and the expression reached its peak in day 2 and decreased thereafter.
Conclusion:
These results demonstrate that IL-1 alpha involves in osteoclast formation induced by 1,25(OH)2D3. And we propose IL-1 alpha may stimulate the proliferation of osteoclast progenitor during the first 4 days of total 6 days culture period.
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