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[A study of histopathology and cell proliferation in calcifying odontogenic cyst]
Objective:
To investigate the histopathologic and clinical characteristics and proliferative activities of calcifying odontogenic cyst (COC).
Methods:
25 cases of COC were reviewed. The expression of proliferating cell nuclear antigen (PCNA) was studied using avidin-biotin peroxidase method and the number of nuclear organizing regions was calculated by means of the argyrophil staining technique in these cases.
Results:
The cases were divided into 13 (52%) cysts and 12 (48%) neoplasms. Of the 12 neoplasms, 4 were odontogenic ghost cell carcinoma, with a PCNA labelling index (65.9% +/- 7.3%) significantly higher than that of benign neoplastic variant (45.8% +/- 11.5%, P < 0.05) and cystic variant (29.3% +/- 11.2%, P < 0.01). The AgNOR counts was higher in the carcinomatous than benign or cystic variant.
Conclusion:
The lesions show considerable diversity in structure and behavior. It is therefore proposed that quantiation of staining for NOR-associated proteins and the PCNA labelling index are diagnostically useful in COC.