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[Effect of bovine plasma fibronectin on rat papilla cells in vitro]
Objective:
To observe the effect of exogenous fibronectin (FN) on the rat dental papilla tissues and cells.
Methods:
Tooth germs of first mandibular molars obtained from 17-day-old rat (SD) embryos were cultured for 3, 6, 9 days on millipore filters in DMEM supplemented with 10% FCS, 50 mg/L Vitamin c. The dental papilla were isolated by trypsin treatment of homologous tooth germs and cultured on uncoated (control) and FN coated millipore filters for 3, 6 days, bovine plasma FN deposited on millpore filters by treated for 2 hours with 80 mg/L FN.
Results:
As shown by histological and cytological data, some mesenchymal cells differentiated into odontoblasts and deposited predentine in cultured tooth germs. In cultured dental papillas, polarized functional cells were observed in FN coated group, odontoblast-like cells never differentiated in control cultures.
Conclusion:
FN may be involved in odontoblast polarization.
