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Updated: Aug 19, 2026
Colorimetric Analysis of Alkaline Phosphatase Activity in S. aureus Biofilm
Published on: April 12, 2019
[Comparison of sampling methods and their effects on alkaline phosphatase levels in gingival crevicular fluid]
Objective:
To observe the change of alkaline phosphatase (ALP) after repeat sampling and compare the two sampling methods (orifice method and intra-pocket method).
Methods:
Gingival crevicular fluid (GCF) was sampled repeatedly by the interval of 20 minutes, with orifice and intra-pocket method respectively, and the levels of ALP in GCF were determined.
Results:
Mean levels of ALP per site and concentration of ALP are not statistically different between two samples after repeat sampling with-orifice method as well as intra-pocket method (P > 0.05). ALP levels (per site and per microliter) are much higher for intra-pocket method than orifice method (P < 0.001). From the viewpoint of the numbers of recovery sites after repeat sampling, though ALP levels at most sites can't entirely recover to their original levels with both method, intra-pocket method is still a little better than orifice method, and ALP levels per site is better than ALP concentration.
Conclusion:
The interval of repeat sampling should be prolonged more than 20 minutes. It is better to determine the GCF-ALP with intra-pocket method and also the recommended pattern that express enzyme levels is total activity per site but not concentration.
