Related Experiment Videos
[The effect of cyclooxygenase-2 inhibitor on acute lung injury]
1Institute of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University, Changqing 400037.
Objective:
To investigate changes of cyclooxygenase-2 (COX-2) mRNA expression and intervention effect of selective cyclooxygenase-2 inhibitor Meloxicam on acute lung injury (ALI).
Methods:
We measured COX-2 mRNA expression by reverse-transcription polymerase chain reaction in rat lung with ALI induced by lipopolysaccharide, and observed changes of prostaglandins (PGs), PaO2 and histopathology.
Results:
Resting rat lung expressed a little COX-2 mRNA; expression of cyclooxygenase-2 mRNA was up-regulated significantly in the lung after rats were injured by LPS. Selective COX-2 inhibitor Meloxicam alleviated histopathologic damage in the lung, inhibited production of PGs and attenuated PaO2 decline.
Conclusions:
COX-2 is a main isoform responsible for enhancing PGs production during ALI. Meloxicam can inhibit activity of COX-2 and may be useful in the treatment of ALI.