Isolation of genes related to blood glucose-control in rat skeletal muscle
Related Experiment Video
Updated: Mar 2, 2026
Measurement of Insulin- and Contraction-Stimulated Glucose Uptake in Isolated and Incubated Mature Skeletal Muscle from Mice
Published on: May 16, 2021
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS & PUMC, Beijing 100005, China.
Objective:
To isolate genes related to blood glucose-control using Sprague Dawley (SD) rat skeletal muscle.
Methods:
Differential gene expression between glucose stimulated and non-glucose stimulated SD rat skeletal muscle was obtained by the differential display (DD) method, Slot blotting hybridization and Northern blot hybridization.
Results:
Several new genes that are differentially expressed in glucose stimulated and non-glucose stimulated SD rat skeletal muscle were isolated. 74 were verified by slot analysis from 181 gene tags isolated. Of them, 33 were cloned and sequenced, and homologous analysis and application for GenBank Access Number were carried out. 21 expressed sequence tag (EST) representing novel genes was confirmed by Northern blot analysis. A total of 9 novel genes showed significant differential expression patterns.
Conclusions:
Using improvements and modifications of the differential display technique, a labor- and cost-saving route was used to identify new genes related to blood glucose-control. We investigated differentially expressed genes at the whole body level instead of the culture cell level, to ensure experimental results closer to the normal physiological state. This technique may be valid in wide-spread application to other related research.
