Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear transfer technologies: between successes and doubts.

J P Renard1, Qi Zhou, D LeBourhis

  • 1Biologie du Développement et Biotechnologies, INRA, 92170, Jouy en Josas, France. renard@jouy.inra.fr

Theriogenology
|January 5, 2002
PubMed
Summary

Nuclear transfer in mammals often leads to developmental issues and animal losses due to epigenetic alterations. Improving cloning efficiency requires understanding and mimicking normal embryonic epigenetic reprogramming.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of extracellular vesicles at parturition in dairy cows with late-gestation heat stress.

JDS communications·2026
Same author

Late-gestation heat stress alters placental structure and function in multiparous dairy cows.

Journal of dairy science·2024
Same author

Uterine wound healing after caesarean section: A systematic review.

European journal of obstetrics, gynecology, and reproductive biology·2024
Same author

PIPAC nebulizer: How to test the new devices in the market, expert recommendations.

Journal of visceral surgery·2022
Same author

Assessment of placental perfusion using contrast-enhanced ultrasound: A longitudinal study in pregnant rabbit.

Theriogenology·2022
Same author

Estimation of milk production in suckling mares and factors influencing their milk yield.

Animal : an international journal of animal bioscience·2022

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Epigenetics

Background:

  • Mammalian cloning via nuclear transfer frequently results in compromised embryo development and high rates of fetal/postnatal losses.
  • These losses are linked to long-term effects from nuclear transfer procedures, including chromatin remodeling and gene deregulation.

Purpose of the Study:

  • To investigate the role of time-dependent DNA methylation in epigenetic alterations post-nuclear transfer.
  • To explore strategies for designing nuclear transfer procedures that better mimic natural embryonic epigenetic remodeling.
  • To enhance the understanding of epigenetic controls in nuclear activities for improved cloning efficiency.

Main Methods:

  • Analysis of epigenetic remodeling in reconstructed embryos.

Related Experiment Videos

  • Screening strategies to optimize nuclear transfer protocols.
  • Investigating the tolerance of early mammalian development to epigenetic abnormalities.

    • Main Results:

    • Nuclear transfer often leads to gene deregulation due to incomplete chromatin remodeling by recipient cytoplasm.
    • Time-dependent DNA methylation events are implicated in these epigenetic alterations.
    • Early development shows some tolerance to epigenetic abnormalities, suggesting potential for altered but functional genomes.

    Conclusions:

    • Improving nuclear transfer efficiency necessitates a deeper understanding of epigenetic reprogramming processes.
    • Pre-conditioning donor cells may enhance cloning success by optimizing epigenetic modifications.
    • Minimizing aberrant phenotypes is crucial for animal welfare in routine nuclear transfer applications.