Related Experiment Videos
Differentiation of human hepatoma Hep G2 cells induced by 10-hydroxycamptothecin
1Department of Pharmacology, Shanghai Institute of Material Medical, Chinese Academy of Sciences, Shanghai 200031, China.
Chinese Medical Journal
|January 5, 2002
Abstract
Objective:
To study the differentiation-inducing effect of hydroxycamptothecin (HCPT) on hepatoma Hep G2 cells.
Methods:
3-[4, 5-dimethylthial-2-yl]-3, 5-diphenyl-tetrazolinium bromide (MTT) colorimetric method was used for determining HepG2 cells viability. Alphafetoprotein (AFP) and albumin (ALB) levels were determined with Elisa Kits.
Results:
The AFP secretion of HCPT-treated cells was reduced by 44.0%-49.2% while the ALB level was increased markedly.
Conclusion:
Hydroxycamptothecin can induce human hepatoma Hep G2 cells to differentiation.