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Updated: Oct 3, 2026

Three-dimensional Quantification of Intestinal Mucus Using Whole-mount Tissue Imaging
Published on: September 12, 2025
Inhibitory effects of intestinal mucus on bacterial adherence to cultured intestinal epithelial cells after surface
1Research Institute of Burn, Southwest Hospital, Third Military Medical University, Chongquing 400038, China.
Objective:
To investigate the relationship between intestinal mucus IgA content and mucus barrier function after surface burns.
Methods:
Detection of IgA content in mucus was performed by enzyme linked immunosorbent assay (ELISA) at different time points after burns. Bacterial adherence to cultured epithelial cells (IEC-6) in vitro using E. coli was assessed for each group.
Results:
The intestinal mucus barrier function declined, parallel to a decrease in IgA content after surface burn in mice. In the normal control group, mucus IgA content was 2.32 D lambda, and 2.51, 1.76, 1.49, 1.06 D lambda at 0.5 h, 1 h, 6 h and 24 h after burn, respectively. Bacterial adherence rate was 0.53 in control group, and 0.46, 0.69, 0.58, 0.81 at 0.5 h, 1 h, 6 h, 24 h after burn, respectively.
Conclusion:
The decrease of intestinal mucus IgA contents is one of the reasons why intestinal mucus barrier function declines after burns.
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