Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transient retinal ischemia-reperfusion in rats.

Y Zhou1, L E Lindner, G C Chiou

  • 1Department of Ophthalmology, Tongren Hospital, Beijing 100730, China.

Chinese Medical Journal
|January 5, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anti-uveitis and inhibition of fibroblast-like corneal and conjunctival cells by interleukin-1 blockers.

Inflammopharmacology·2007
Same author

Revascularisation in diabetics with multivessel coronary artery disease.

Heart (British Cardiac Society)·2004
Same author

Anatomy of coronary disease in diabetic patients: an explanation for poorer outcomes after percutaneous coronary intervention and potential target for intervention.

Heart (British Cardiac Society)·2004
Same author

Explorations of a trust approach for nursing ethics.

Nursing inquiry·2002
Same author

Effects of N-nitropyrazoles on ocular blood flow of rabbits and retinal function recovery of rat eyes after ischemic insults.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2002
Same author

Review: effects of nitric oxide on eye diseases and their treatment.

Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics·2001

Short retinal ischemia-reperfusion in rats induces retinal IL-1 beta and neuronal cell apoptosis. However, this cell death does not significantly impact overall retinal function.

Area of Science:

  • Ophthalmology
  • Neuroscience
  • Immunology

Background:

  • Retinal ischemia-reperfusion injury is a significant concern in various ocular conditions.
  • Understanding the cellular and molecular mechanisms of early retinal damage is crucial for developing therapeutic strategies.

Purpose of the Study:

  • To investigate the impact of brief transient retinal ischemia-reperfusion on retinal neuronal cell death and inflammatory markers in a rat model.
  • To quantify the levels of Interleukin-1 beta (IL-1 beta) and Tumor Necrosis Factor alpha (TNF alpha) following ischemic insult.

Main Methods:

  • Retinal ischemia-reperfusion was induced in rats by elevating intraocular pressure for 1 or 5 minutes.
  • Neuronal cell apoptosis was assessed using the TUNEL assay and light microscopy.
  • Retinal IL-1 beta and TNF alpha levels were measured using enzyme-linked immunosorbent assay (ELISA).

Related Experiment Videos

Main Results:

  • Transient retinal ischemia-reperfusion led to a significant increase in TUNEL-positive cells, indicating apoptosis in various retinal layers.
  • Retinal IL-1 beta levels were significantly elevated at 6 hours post-reperfusion.
  • No significant increase in retinal TNF alpha was observed, and leukocyte infiltration was minimal.

Conclusions:

  • Even short durations (1-5 minutes) of transient retinal ischemia-reperfusion can trigger retinal neuronal cell apoptosis and upregulate IL-1 beta.
  • The observed apoptosis, while significant at the cellular level, did not appear to compromise overall retinal function in this model.