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Enhanced contact hypersensitivity in human monocyte chemoattractant protein-1 transgenic mouse
N Mizumoto1, K Iwabichi, H Nakamura
1Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan.
Immunobiology
|January 5, 2002
Summary
Monocyte chemoattractant protein-1 (MCP-1) enhances contact hypersensitivity (CHS) by increasing Langerhans
Area of Science:
- Immunology
- Cell Biology
- Dermatology
Background:
- Monocyte chemoattractant protein-1 (MCP-1) is a cytokine that attracts monocytes, T cells, and dendritic cells.
- The exact role of MCP-1 in immune responses is not fully understood.
Purpose of the Study:
- To investigate the role of MCP-1 in contact hypersensitivity (CHS).
- To analyze the effects of MCP-1 on Langerhans' cells (LC) during CHS.
Main Methods:
- Used human MCP-1 transgenic mice (hMCP-1Tgm) and wild-type mice.
- Administered 2,4-dinitrofluorobenzene (DNFB) or fluorescein isothiocyanate (FITC) for sensitization.
- Analyzed LC migration, size, and expression of I-Ad and B7-1.
- Used anti-hMCP-1 antibodies and recombinant hMCP-1 (rhMCP-1) in experiments.
Main Results:
- hMCP-1Tgm mice showed enhanced CHS compared to non-transgenic mice.
- Anti-hMCP-1 antibodies inhibited CHS in hMCP-1Tgm mice.
- MCP-1 accelerated LC migration to draining lymph nodes and increased their expression of I-Ad and B7-1.
- rhMCP-1 administration mimicked these effects in wild-type mice.
Conclusions:
- MCP-1 enhances CHS by promoting LC migration and up-regulating their immune-stimulatory molecules.
- MCP-1 plays a significant role in T cell activation during CHS.
