Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

Source And Potency Of Stem Cells

Source And Potency Of Stem Cells

Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimising same day discharge hemithyroidectomy: defining outcomes, unplanned admissions and patient experience.

Annals of the Royal College of Surgeons of England·2025

Same author

Development of a novel Bluetooth Low Energy device for proximity and location monitoring in grazing sheep.

Animal : an international journal of animal bioscience·2024

Same author

Genome-wide association studies of parasite resistance, productivity and immunology traits in Scottish Blackface sheep.

Animal : an international journal of animal bioscience·2024

Same author

Thyroid lobectomy for low-risk thyroid cancers.

Annals of the Royal College of Surgeons of England·2022

Same author

Metastatic seminoma with isolated gastric metastases: a case report.

Scottish medical journal·2019

Same author

New mastitis phenotypes suitable for genomic selection in meat sheep and their genetic relationships with udder conformation and lamb live weights.

Animal : an international journal of animal bioscience·2018

Search research articles

Related Experiment Video

Updated: Jul 23, 2026

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Pluripotential stem cells derived from migrating primordial germ cells.

G Durcova-Hills¹, J Ainscough, A McLaren

¹Wellcome/CRC Institute, Cambridge, UK.

Differentiation; Research in Biological Diversity

|January 5, 2002

Summary

New embryonic germ cell (EGC) lines derived from mouse primordial germ cells (PGCs) show pluripotency and normal karyotype. Imprint erasure for key genes occurs before 9.5 days post coitum in male germ cells.

More Related Videos

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

Related Experiment Videos

Last Updated: Jul 23, 2026

Isolation and Derivation of Mouse Embryonic Germinal Cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Published on: October 22, 2009

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Differentiation of Newborn Mouse Skin Derived Stem Cells into Germ-like Cells In vitro

Published on: July 16, 2013

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

Area of Science:

Developmental biology
Stem cell research
Epigenetics

Background:

Embryonic germ cells (EGCs) are pluripotent stem cells derived from primordial germ cells (PGCs).
Previous studies have successfully derived EGCs from mouse PGCs at various developmental stages.
Understanding the properties and developmental potential of EGCs is crucial for regenerative medicine and developmental studies.

Purpose of the Study:

To derive and characterize new embryonic germ cell (EGC) lines from migrating mouse primordial germ cells (PGCs) at 9.5 days post coitum (dpc).
To assess the developmental potency and epigenetic status, specifically DNA methylation patterns of imprinted genes, in these newly derived EGC lines.

Main Methods:

Derivation of EGC lines from 9.5 dpc mouse embryos.
Characterization of EGCs for pluripotency markers (colony morphology, alkaline phosphatase, SSEA-1) and karyotype analysis.
In vivo assessment of developmental potency using fetal chimera assays.
Analysis of DNA methylation status of imprinted genes (Igf2r, p57Kip2, Lit1, H19, Igf2) using methylation-sensitive techniques.

Main Results:

Four male EGC lines were successfully derived from 9.5 dpc migrating PGCs, all exhibiting normal karyotypes and typical EGC properties.
In vivo studies demonstrated that two EGC lines contributed to various tissues in fetal chimeras.
Epigenetic analysis revealed that Igf2r, p57Kip2, and Lit1 were unmethylated, while H19 and Igf2 showed hypo-methylation in the 9.5 dpc EGC line compared to 11.5 dpc EGC lines.

Conclusions:

Newly derived 9.5 dpc male EGC lines possess pluripotency and developmental potential.
The findings suggest that imprint erasure in the male germ line for the examined imprinted genes occurs before 9.5 dpc.