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Updated: Oct 3, 2026

Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
Published on: June 16, 2023
[Changes of calcitonin gene-related peptide content in induced sputum from patients with COPD and asthma]
Objective:
To explore the role of sensory neuropeptide calcitonin gene-related peptide (CGRP) in the pathogenesis of chronic airway inflammatory diseases COPD and bronchial asthma.
Methods:
Patients with COPD (n = 19), bronchial asthma (n = 14), all were in stable stage and 10 normal volunteers were examined. After hypertonic saline inhalation challenge in all subjects, CGRP-LI concentration in the induced sputum was measured by radioimmunoassay. Cellular content was assayed by microscopic analysis, the relation between CGRP-LI level and FEV1 value was calculated by linear regression.
Results:
The sputum CGRP concentrations in patients with COPD and patients with asthma were (15.97 +/- 2.15) ng/L, (18.79 +/- 3.91) ng/L, respectively, both were significantly higher than those in normal volunteers (2.36 +/- 0.35) ng/L. Moreover, CGRP concentrations in induced sputum in each disease group were correlated with the degree of airflow obstruction, r = -0.50 and -0.61, respectively (P < 0.05). The percentage of neutrophil cell count (64.9 +/- 2.9)% was significantly higher in patients with COPD (P < 0.01), while the percentage of eosinophil cell count (5.8 +/- 0.5)% was increased in patients with asthma (P < 0.01).
Conclusions:
The data suggested that CGRP release may participate in the chronic inflammation of patients with COPD and bronchial asthma.
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