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[Cloning and partially sequencing of mouse dentin sialophosphoprotein encoding mature protein]
Objective:
Cloning and partially sequencing of mouse dentin sialophosphoprotein (DSPP) encoding mature protein.
Methods:
Total RNA was extracted from the tooth germs of newborn mouse by acid guanidinium thiocyanata-phenol-chloroform method, the desired DNA product was obtained from the total RNA by RT-PCR with the primers including Oligo(dt) and two gene specific primers. The segment (about 3 Kbp) was inserted into pBluescript vector and the interesting plasmid was transformed into E. Coli host strain XL1-Blue. The double-stranded DNA of the positive clone was analyzed by restriction endonuclease mapping and DNA sequencing.
Results:
The restriction endonuclease map and sequence of mouse DSPP encoding mature protein were consistent with those of the published.
Conclusion:
The mouse DSPP cDNA encoding mature protein was obtained for further study.