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Updated: Aug 21, 2026

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
[Collagen-binding proteins of human oral spirochetes]
1Research Institute of Stomatology, Shanghai Second Medical University, Shanghai 200011.
Objective:
To evaluate pathogenic effect of the collagen-binding proteins(CBP) of human oral spirochetes.
Methods:
Immunoblot analysis, immunoelectron microscopy and other experiments had been used.
Results:
It was showed that: type I, IV and V CBP were detected in heated and unheated preparations from Treponema denticola(Td) ATCC 33520 and Treponema socranskii (Ts) ATCC 35534. Few CBP, however, were detected in heated and unheated preparations from a recently characterized isloate, Treponema medium(Tm) strain G7201. 27,000 type V CBP of Td, 110,000 type I of Ts and 95,000 type IV of Tm were located on the envelopes of the individual cells. The adherence of Td to the collagen-coated surface was significantly greater than that of Tm.
Conclusion:
The CBP on the oral spirochetal cells play an important role in their adherence to collagen-rich connective tissues of the host.
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