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Updated: Aug 9, 2026

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
Combined use of LC/MS and a biological test for rapid identification of marine mycotoxins produced by Trichoderma
Anne Landreau1, Yves François Pouchus, Claire Sallenave-Namont
1ISOMer, SMAB-URM11 (Toxinologie marine), Faculté de Pharmacie, Université de Nantes, BP 53508, 44035 Nantes Cedex 01, France. Anne.Landreau@sante.univ-nantes.fr
Abstract:
Trichoderma koningii Oudemans, a strain isolated from a shellfish farming area, was selected for its high frequency in samples and its ability to produce metabolites when cultured in natural seawater. Combined use of LC/MS and a biological test on blowfly larvae allowed the characterization of four compounds after purification in only two steps (VLC and HPLC). ESI/MS, a powerful tool for rapid identification and sequence determination of peptides, confirmed that these compounds were peptide, alpha-aminoisobutyric acid and amino alcohol (peptaibols), the usual metabolites of Trichoderma.
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