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Updated: Oct 3, 2026

Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
[Expression in E. coli of protein encoded by human esophageal cancer related gene-1]
1Cancer Institute (Hospital), Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021, China.
Objective:
To express esophageal cancer related gene-1 (ECRG-1)--in E. coli.
Methods:
The human ECRG-1 was cloned by RT-PCR. Expression plasmid of the ECRG-1/GST pi fusion gene was constructed and introduced into E. Coli. The fusion protein was induced to express by ITPG. The recombinant protein was identified by Western blot. BALB/C mice were immunized with the protein purified by SDS-PAGE for the preparation of polyclonal antibody.
Results:
DNA sequencing confirmed that the sequence of ECRG-1 was identical to that of the previously obtained by 5'-RACE technique. Expression of the protein in E. coli was verified by Western blot. The polyclonal antibody obtained from immunized BALB/C mice had a title of 1:3,200.
Conclusion:
The antibody available is useful for the further study of structure and function of the esophageal cancer related protein.
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