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Updated: Oct 3, 2026
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
[Modulating effect of vitamin D3 in vitro on EGFR mRNA expression of human breast cancer cell lines]
1Department of Pathology, Shanghai Sixth People's Hospital, Shanghai 200233, China.
Objective:
To assess the modulating effects of vitamin D3, combined with or without tamoxifen, on cell proliferation and EGFR mRNA level of human breast cancer.
Methods:
Two breast cancer cell lines (MCF-7 and ZR-75-1) were treated with various concentrations of vitamin D3, alone or in combination with tamoxifen, for 6 days. Cell proliferation was assessed by MIT uptake and EGFR mRNA level by Northern blot.
Results:
10(-10)-10(-7) mol/L vitamin D3 strongly inhibited proliferation of the 2 breast cancer lines, while 10(-9)-10(-7) mol/L tamoxifen stimulated proliferation of MCF-7 cell. The growth inhibitory effect on the 2 cell lines could be augmented and accompanied by a decrease in EGFR mRNA level after combined treatment with IC50 dose of tamoxifen and 10(-10)-10(-9) mol/L vitamin D3.
Conclusion:
Vitamin D3 can be considered as a useful anti-tumor drug in the treatment of breast cancer.
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